Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • saha
    Junior Member
    • Jan 2010
    • 5

    #1

    Mapping to SOLiD reads to draft genome

    I am presently involved in hybrid assembly and want to map my SOLiD reads against ROCHE contigs (length more than 1000bp). I approach is to map the reads with corona_lite, but facing problem with the cmap file. I found a small paragraph regarding this particular situation in corona_lite documentation but it is not that illustrative. Usually for mapping against human genome we take cmap as follows:

    1 1 path to reference for each chr. path to the double encoded file

    But for a draft genome i am confused. it tried with this following approach but i failed. i present cmap that i tried was:

    1 contig0001 path to the multifasta contig file

    Any help and guidance will be appreciable.

    with regards,
    saha
  • westerman
    Rick Westerman
    • Jun 2008
    • 1104

    #2
    Either make a very large cmap file with one line per contig file. Or put all of the contigs into one file and separate them by 'N's; that is the file will look like one large sequence. I usually do the latter. If you are clever with how many N's you use to separate each contig then you can get information from the mapping file; e.g., map to bases 1-1323 means contig#1, bases 1500-2300 contig #2, etc.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    34 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    25 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    41 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    45 views
    0 reactions
    Last Post SEQadmin2  
    Working...