Y-Based SOLiD Adaptors
Has anyone heard if AB was planning on switching to Y-based adaptors and A- tailing for fragment libraries?
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Yes, but you should contact ABI since it is not officaly released yet...
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Does anybody know the sequence of the multiplexing adapters (barcoding) for the SOLiD system?
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there are a few service providers out there. Your best bet would be to contact ABI. Seqwright and Agencourt are the two big ones, but I wouldnt send my samples anywhere other than agencourt.
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Here are the sequences for most of the current solid oligos, I tried to display it like the post in the solexa forum. I don't know the sequences of the P1 primer on the bead, the actual sequencing primers, or all the "block" oligos.
SOLiD 2.0 Oligos
P1 Adapter:
5' -----------------CC ACTACGCCTCCGC TTTCCTCTCTATGGGCAGTCGGTGAT (-) -------------------- -------------------- - 3'
3' ---------------TTGG TGATGCGGAGGCG AAAGGAGAGATACCCGTCAGCCACTA (-) -------------------- -------------------- - 5'
P2 Adapter:
5' -------------------- -------------------- ------------------ (-) AGAGAATGAGGAACCCGGGG CAGTT--------------- - 3'
3' -------------------- -------------------- ------------------ (-) TCTCTTACTCCTTGGGCCCC GTC----------------- - 5'
Library PCR Primer1:
5' -----------------CC ACTACGCCTCCGC TTTCCTCTCTATG------------- (-) -------------------- -------------------- - 3'
3' -------------------- -------------------- ------------------ (-) -------------------- -------------------- - 5'
Library PCR Primer2:
5' -------------------- -------------------- ------------------ (-) -------------------- -------------------- - 3'
3' -------------------- -------------------- ------------------ (-) TCTCTTACTCCTTGGGCCCC GTC----------------- - 5'
Resulting Frag Library:
5' -----------------CC ACTACGCCTCCGC TTTCCTCTCTATGGGCAGTCGGTGAT (N) AGAGAATGAGGAACCCGGGG CAG----------------- - 3'
3' -----------------GG TGATGCGGAGGCG AAAGGAGAGATACCCGTCAGCCACTA (N) TCTCTTACTCCTTGGGCCCC GTC----------------- - 5'
Mate pair oligos
EcoP15I Cap Adapter (note AC 3' overhang on one end)
5' -------pCTGCTGTAC---------- - 3'
3' --------GACGACAp----------- - 5'
Internal Adapter (note: lowercase T in top strand has internal biotin, note GT 3' overhangs for sticky end circularization)
5' --------CGTACAtCCGCCTTGGCCGT----- - 3'
3' ------TGGCATGTAGGCGGAACCGG------- - 5'Last edited by universalprimer; 09-28-2008, 12:29 PM.
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SOLiD Adapters
Hello,
I would like to sequence gene expression tags with the SOliD machine-does anybody have the adapter-sequences flanking the inserts or do you know where could I get them?
Also, is there a relaible service provider for the SOliD system out there?
Thank you very much in advance!Tags: None
- Stuck
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