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  • mulligan
    replied
    Y-Based SOLiD Adaptors

    Has anyone heard if AB was planning on switching to Y-based adaptors and A- tailing for fragment libraries?

    Leave a comment:


  • Chipper
    replied
    Yes, but you should contact ABI since it is not officaly released yet...

    Leave a comment:


  • atp
    replied
    Does anybody know the sequence of the multiplexing adapters (barcoding) for the SOLiD system?

    Leave a comment:


  • snetmcom
    replied
    there are a few service providers out there. Your best bet would be to contact ABI. Seqwright and Agencourt are the two big ones, but I wouldnt send my samples anywhere other than agencourt.

    Leave a comment:


  • exprofiler
    replied
    thank you !!

    Leave a comment:


  • universalprimer
    replied
    Here are the sequences for most of the current solid oligos, I tried to display it like the post in the solexa forum. I don't know the sequences of the P1 primer on the bead, the actual sequencing primers, or all the "block" oligos.

    SOLiD 2.0 Oligos

    P1 Adapter:
    5' -----------------CC ACTACGCCTCCGC TTTCCTCTCTATGGGCAGTCGGTGAT (-) -------------------- -------------------- - 3'
    3' ---------------TTGG TGATGCGGAGGCG AAAGGAGAGATACCCGTCAGCCACTA (-) -------------------- -------------------- - 5'

    P2 Adapter:
    5' -------------------- -------------------- ------------------ (-) AGAGAATGAGGAACCCGGGG CAGTT--------------- - 3'
    3' -------------------- -------------------- ------------------ (-) TCTCTTACTCCTTGGGCCCC GTC----------------- - 5'

    Library PCR Primer1:
    5' -----------------CC ACTACGCCTCCGC TTTCCTCTCTATG------------- (-) -------------------- -------------------- - 3'
    3' -------------------- -------------------- ------------------ (-) -------------------- -------------------- - 5'

    Library PCR Primer2:
    5' -------------------- -------------------- ------------------ (-) -------------------- -------------------- - 3'
    3' -------------------- -------------------- ------------------ (-) TCTCTTACTCCTTGGGCCCC GTC----------------- - 5'

    Resulting Frag Library:
    5' -----------------CC ACTACGCCTCCGC TTTCCTCTCTATGGGCAGTCGGTGAT (N) AGAGAATGAGGAACCCGGGG CAG----------------- - 3'
    3' -----------------GG TGATGCGGAGGCG AAAGGAGAGATACCCGTCAGCCACTA (N) TCTCTTACTCCTTGGGCCCC GTC----------------- - 5'


    Mate pair oligos

    EcoP15I Cap Adapter (note AC 3' overhang on one end)
    5' -------pCTGCTGTAC---------- - 3'
    3' --------GACGACAp----------- - 5'

    Internal Adapter (note: lowercase T in top strand has internal biotin, note GT 3' overhangs for sticky end circularization)
    5' --------CGTACAtCCGCCTTGGCCGT----- - 3'
    3' ------TGGCATGTAGGCGGAACCGG------- - 5'
    Last edited by universalprimer; 09-28-2008, 12:29 PM.

    Leave a comment:


  • exprofiler
    started a topic SOLiD Adapters

    SOLiD Adapters

    Hello,
    I would like to sequence gene expression tags with the SOliD machine-does anybody have the adapter-sequences flanking the inserts or do you know where could I get them?
    Also, is there a relaible service provider for the SOliD system out there ?
    Thank you very much in advance!

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