[KevinLam]

From the In Sequence article, link, but login is required:

"Real-time data processing means no compute cluster is necessary."

Any idea how this could be possible when a cluster is required to run a solid v4 and it does data processing on the fly, if not exactly "in real time"?

--
Phillip

I recall speculation that V4 to HQ cannot be upgraded as its entirely different.
In the preview launch they did allude to the fact that instead of slides it is now 6 lanes of microfluidic on a flowchip (which still looks like a slide to me though)

and the highly advertised fact that "Developed and manufactured with long-standing partner Hitachi High-Technologies, the 5500xl delivers on the promise of the SOLiD 4hq"

So based on this I am guessing the optics and fluidics are changed. Hence perhaps that's the main reason behind not requiring a on instrument cluster.

however, if you asked me, I really feel that the cluster was provisioned so that users may run at least bare minimal analysis on the data, without having to buy an offline cluster. The running time of the cycle scans are probably long enough to process the images with a desktop computer. granted it has enough storage.

just my two cents.
btw for those that do not have access to genomeweb try the official press release.

http://www.lifetechnologies.com/news-gallery/press-releases/2010/life-techologies-lauches-ew-solid-sequecer-to-drive-advaces-i--c.html

[pmiguel]

From the In Sequence article, link, but login is required:

"Real-time data processing means no compute cluster is necessary."

Any idea how this could be possible when a cluster is required to run a solid v4 and it does data processing on the fly, if not exactly "in real time"?

--
Phillip

[Chipper]

Looks promising. Up to 230 M fragments per lane and possibility to run 1 lane in one day.

* Pay-per-use reagents for cost-effective
consumable usage

* 1 day for 35 bp, 1 lane

* Nanobeads: Up to 300 Gb or greater than 4.8 B tags (paired-end or
mate-paired runs)

* Reference-free analysis with Exact Call Chemistry Module

[truthseqr]

SOLiD @ ASHG

AB is premiering two new instruments at ASHG next week.

Mobile ASHG calendar: http://m.appliedbiosystems.com/ashg/

Twitter account: @SOLiDSequencing

SOLiD Community: http://solid.community.appliedbiosystems.com/

More info soon at: solid.appliedbiosystems.com/solid5500/

[SrScientist]

regarding "performance of Ion Torrent" in that seminar they´ve also mentioned that the accuracy of the PGM is increasing and could reach q40 at launch (e.coli; ~2-6x coverage).
In terms of speed one can go for up to 4 runs per day.
Application number will increase too because of the R&D-man-power behind LifeTech compared to Ion Torrents.

[KevinLam]

Oh there was a brief mention of the Ion Torrent at the presentation as well but nothing of great significance. I do wish they marketing fellows will push ion torrent out faster but i think they are trying to streamline production by testing if invitrogen kits can replace the ones at ion torrent. I do hope they do not sacrifice compatibility over performance

[KevinLam]

I have seen the presentation as well.
They do mention it is upgradeable although I think its more like a trade in

I DO NOT see the computer cluster anymore, that's something I am curious about.
it is named 5500XL and 5500 solid system
which is your 4 HQ and PI

One can only fathom their obession with these 4 digits judging by similar instruments named
AB Sciex Triple Quad 5500 and the AB Sciex QTrap 5500

Honestly the 5500 numbers are of no numerical significance AFAIK.

Finally we are at 75 bp though

[SrScientist]

I´ve seen that presentation about these new instruments now too. Indeed, two new instruments. Cooperation with Hitachi. Nice robotics; much faster than previous solids; new slides (more flexible); higher accuracy (and keeps on increasing); and more.

[daughart]

A little bird told me that the SOLiD 4HQ will NOT be an upgrade, it will be a completely new machine. I suspect that the computing hardware will remain the same (basically the bottom half of the main machine and the computer module, which holds some buffers in a cabinet below), but the top half of the sequencing machine will be completely replaced. I have heard word of improvements to the slide loading system and other improvements to fix slide issues and fluidics. New imaging, new robotics.

EDIT: Also, first post!! Been reading seqanswers for the last 18 months, it's been really helpful!

[pmiguel]

A haiku for Phillip:

Another benefit of dumping the "solid" brand for a numeric one. Impossible to write haikus about it.

[pmiguel]

Sorry Phillip (go Boilers!), but I'm not joking or mistaken. During the talk of the Life Tech. sequencing platforms, there was a slide showing both instruments w/ the new names, and he said that we were one of the first groups of people to hear that new info. The speaker was Michael Rhodes, Senior Manager - Sequencing, from Life Technologies.

Well, I still have my doubts about the SOLiD being "de-branded" to be called something like the "5500". Not that I would necessarily mind. This "brand" meme that hit industry pundit around a decade ago, is now impacting Purdue. In fact, last weekend was the launch of our new brand-thing "makers-all". Some sort of post-modern play on "boilermakers". Probably would need an introductory course in semiotics to understand all that is in play here. But I digress...

--
Phillip

[westerman]

A haiku for Phillip:

Solid. Misery.
Crashing. Cracking. Sliding. Pain!
Get better? Some day.

[pmiguel]

Yes, 1 slide for Pi, 2 for HQ. I don't recall any other specific differences. I believe they both use the same chemistries and software.

I thought HQ was to be using 0.7 micron beads instead of the current 1 micron beads. Hence, more beads per slides. But also necessitating new optics (camera?) capable of resolving the smaller beads. And, for those of us stuck with pre-HQ instruments, a $100K bill to upgrade.

Chemistry-wise, I understood they would be offering a new 5-base encoding chemistry that could be used along with the normal 2-base encoding chemistry to allow conversion to base space reads with out the normal error propagation issues. (Or, at least, the errors would propagate a maximum of 5 bases.)

No word on either of those capabilities?

--
Phillip

[RCJK]

Yes, 1 slide for Pi, 2 for HQ. I don't recall any other specific differences. I believe they both use the same chemistries and software.

[Chipper]

Good to hear that they decided to use the new platform for HQ as well, any info on the difference between the two? One slide for PI and two for HQ?