Hi,
I am working with small cell number ChIP-seq, and was wondering if anyone has any experience doing a WGA or similar after the ChIP step, before going on to make the library and seq?
I have tried doing the Illumina PCR amplification protocol after attaching the adapters, but it results in a lot of bias based on size and GC content, which for small samples basically kills the specificity of the experiment.
I'm considering the WGA kit from Sigma... any experience/guesses on whether that might work better? I'm also open to other kits people have used.
Thanks in advance!
I am working with small cell number ChIP-seq, and was wondering if anyone has any experience doing a WGA or similar after the ChIP step, before going on to make the library and seq?
I have tried doing the Illumina PCR amplification protocol after attaching the adapters, but it results in a lot of bias based on size and GC content, which for small samples basically kills the specificity of the experiment.
I'm considering the WGA kit from Sigma... any experience/guesses on whether that might work better? I'm also open to other kits people have used.
Thanks in advance!
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