This kit is too expensive. Anyone with knowledge, information, or a friend who might know a guy who once made a home-made version of this kit, please let me know ! Thanks!
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Originally posted by dcyounge View PostThis kit is too expensive. Anyone with knowledge, information, or a friend who might know a guy who once made a home-made version of this kit, please let me know ! Thanks!
Tricky though. I wouldn't touch it unless I had 100+ libraries to make.
Do you need strand specificity? You could just by the Illumina RNA TruSeq kit and substitute SOLiD adapters for the Illumina adapters. Much cheaper! They even throw in the poly A+ RNA purification.
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Phillip
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Agree, just buy the Illumina kit and substitute in your own adapters. The cost of synthesizing the adapters is minimal. We are getting good data with using 1/3 of all the reagents.
On second thought, it's not that simple since the SOLiD adapters are not Y-shaped. I don't really know much about SOLiD library preparation.Last edited by ETHANol; 03-29-2012, 10:10 AM.--------------
Ethan
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Originally posted by ETHANol View PostAgree, just buy the Illumina kit and substitute in your own adapters. The cost of synthesizing the adapters is minimal. We are getting good data with using 1/3 of all the reagents.
Originally posted by ETHANol View PostOn second thought, it's not that simple since the SOLiD adapters are not Y-shaped. I don't really know much about SOLiD library preparation.
Okay, that does mean you have to use SOLiD primers instead of PPC from the Illumina kit to do your enrichment PCR. Still the outlay for oligos will not be too bad.
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Phillip
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Thanks folks ! Found an epicentre kit that will be better for our experiments. But trying to figure out what their secret "degradase" is .. any thoughts ? see Figure 1 http://www.epibio.com/posters/Enhanc...ome_ABRF12.pdf
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Originally posted by dcyounge View PostThanks folks ! Found an epicentre kit that will be better for our experiments. But trying to figure out what their secret "degradase" is .. any thoughts ? see Figure 1 http://www.epibio.com/posters/Enhanc...ome_ABRF12.pdf
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Phillip
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