-
Why are the ends different in RNA seq?
Hi guys,
I have a feeling that everyone but me knows or has figured this out...
Why are the ends different in a RNA seq library? I can see that the both primers anneal in the 3' end to the adaptor sequence and that the genomic sequencing primer only anneal to the long PCR primer, but I can't figure out what prevents the final library to be a random selection of fragments with primer 1.1 or 2.1 in both ends and some with different ends. What am I missing?
Happy sequencing,
Darwin
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Leave a comment: