For those of you familiar with the Pippin Prep, currently used for automated DNA size selection and band capture, your thoughts on whether or not Sage Science should commercialize a series of cassettes for RNA too? What RNA applications are of greatest interest? Thanks for your thoughts and help!
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If these were denaturing gels that would work on strand denatured DNA as well, I think they would be very useful.
Subtext of a recent thread
Bridged amplification & clustering followed by sequencing by synthesis. (Genome Analyzer / HiSeq / MiSeq)
is that short ssDNA/adapters may be annealing to longer library products -- preventing their removal via size-selection on dsDNA products. If the size selection were done on ssDNA there is no where for these short contaminants to "hide" and they should be removed.
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Phillip
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Yes, send them to Purdue and we will test them on our Pippin prep.
I think there are lots of otherwise perfectly good libraries that are getting hosed by small "hitchhiker" amplicons that can't be removed with double-stranded size-selection methods. Maddening!
Well, there is always normal denaturing PAGE. But it always seems like such a hassle to extract the sample from polyacrylamide gels.
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Phillip
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