Clontech Low Input Library Prep Kit

[slees]

Hello,

Anyone used Clontech Low Input Library Prep Kit (Cat. No. 634947)? It is suggested for the lib prep following cDNA synthesis/amplification with the SMARTer RNA-seq kit. But I think it can also be used for ChIP-seq or other lib prep which starts from small amount of DNA. The performance of this kit, according to the manual, is really impressive – it generates library that is enough for Illumina sequencing from 100 pg of dsDNA with only 14 cycles of PCR amplification, and all the reactions are done in a single tube. Is it possible? Does anyone has some clue how this kit works?

Best,
Li

[luc]

I, too, would like to know how it works.

It looks like the clontech product is a re-badged version of the Rubicon Genomics Thruplex kit ( http://rubicongenomics.com/products/thruplex/ ). The manuals are identical.
The Thruplex kit is cheaper, though.

[slees]

Thanks luc! The information you provided is very useful, there is actually a chart on Rubicon Genomics website:

http://rubicongenomics.com/applications/dna-seq/

[luc]

Thanks, Slees!

I had not seen this page.
I have to say this protocol is a thing of beauty (and seeming simplicity).
The patent is very interesting, too.

http://www.google.co.uk/patents/US7803550