We've used the TruSeq PCR-Free kit on very short fragments, 170bp, and are getting a double peak when looking at the results on the bioanalyzer (attached). Has anyone seen this before?
-
-
If the trace is for adapter ligated fragments, this could be due to structure of adapters. You can do a PCR with small aliquot of library and check the size of amplicons.Last edited by nucacidhunter; 08-15-2014, 11:07 PM.
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment