We've used the TruSeq PCR-Free kit on very short fragments, 170bp, and are getting a double peak when looking at the results on the bioanalyzer (attached). Has anyone seen this before?
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If the trace is for adapter ligated fragments, this could be due to structure of adapters. You can do a PCR with small aliquot of library and check the size of amplicons.Last edited by nucacidhunter; 08-15-2014, 11:07 PM.
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