Like a lot of labs, we've replaced spin/vacuum columns with magnetic bead extraction for every part of our library construction protocols. However, the commercial products are expensive. We found some recipes for making your own SPRI bead mix at a tiny fraction of the cost, but they weren't written very well, so here's a new one.
Enjoy!
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UPDATED April 17, 2015: we now have a protocol for RNA beads (comparable to RNAClean XP) as well
UPDATED AGAIN October 17, 2015: there was an error in the RNA bead mix volumes
UPDATED AGAIN October 21, 2015: there was also an error in one of the NaCl concentrations (thanks, blameyourelf!)
FINAL UPDATE February 16, 2015: we've moved the protocol to OpenWetWare for easier updating; please go there for the most recent version
Enjoy!
---
UPDATED April 17, 2015: we now have a protocol for RNA beads (comparable to RNAClean XP) as well
UPDATED AGAIN October 17, 2015: there was an error in the RNA bead mix volumes
UPDATED AGAIN October 21, 2015: there was also an error in one of the NaCl concentrations (thanks, blameyourelf!)
FINAL UPDATE February 16, 2015: we've moved the protocol to OpenWetWare for easier updating; please go there for the most recent version
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