Hi all,
I just got the data back from 7 samples Ive run on the Bioanalyser (see attached file). All samples have been prepared with the illumina Paired-End kit and I size selected the libraries around 350pb (+/- 25bp). In more or less all the samples I see an additional band around 500-550pb.
Basically, I have no idea what this band is since the only step after the size selection is gel extraction with the qiagen kit.
Have any of you ever seen something similar? I was wondering whether it could be somekind of dimers that have been forming?
Any ideas about what it could be, how to test it and how to get rid of it will be greatly appreciated.
Thanks,
Jesper
I just got the data back from 7 samples Ive run on the Bioanalyser (see attached file). All samples have been prepared with the illumina Paired-End kit and I size selected the libraries around 350pb (+/- 25bp). In more or less all the samples I see an additional band around 500-550pb.
Basically, I have no idea what this band is since the only step after the size selection is gel extraction with the qiagen kit.
Have any of you ever seen something similar? I was wondering whether it could be somekind of dimers that have been forming?
Any ideas about what it could be, how to test it and how to get rid of it will be greatly appreciated.
Thanks,
Jesper
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