I'm planning to make libraries from several metagenomic DNA samples using Nextera XT for the purpose of de novo assembly of microbial genomes. I'm unconcerned with complete reconstruction of individual genomes but as I would like to take a "gene-centric" approach to these metagenomes I want to maximize depth to aid in assembly. My question is: given DNA input of constant mass but from samples of very different fragment size distributions (see attached Tapestation traces) how consistent can I expect my library insert sizes to be? I assume that if Nextera chemistry is used for both amplicon and whole genome sequencing that Tn5 activity must be influenced more by template mass than anything but I wanted to ask the community.
Thank you!
Thank you!
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