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I came across this technology for DNA shearing http://www.kbioscience.co.uk/instrum...-DNA_shear.htm Has anyone got any experience with this technology?
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Hi,
We are successfully using a Covaris sonicator to shear DNA for ChIP-Seq on Solexa platform.
Especially the reproducibility is higher than that of other systems we have tested so far.
Only the cost price is a major drawback.
Best
andibody -
Well, buy a SOLiD then, and you will get a Covaris also.
The other drawback is throughput I guess, the Bioruptor takes up to six samples at once.
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I did see they now have a 96well version as well. I'm slightly worried that the biorupters sonication heats the sample and we could get biased shearing. On the Covaris website they state that this method is isothermal. Did anyone check this yet?Comment
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KASPar Kbioscience
Hallo,
does anybody have experience with the KASPar genotying assay from Kbioscience?
I am thinking of using this technology but I first would like to know your experience.
Thanks a lot!Comment
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I'm looking to create a couple of 8kb paired end rapid libraries for 454 seq and don't have a HydroShear, I realize there are a few places to outsource the shearing step to facilities such as Profile Genomics or Covaris but has anyone tried Covaris's g-TUBE single-use device that shears genomic DNA into selected fragments sizes ranging from 6kb to 20kb. The only equipment needed is a compatible bench-top centrifuge, looks like it would be about $27/sample.Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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