Streptavidin Bead Quantitation

[apeters6]

Hi Everyone!

I am trying to evaluate how well our new robot handles bead clean-ups with streptavidin beads. I would like to objectively quantitate bead carry over (after removal of supernatant) rather than just visually assessing. Any ideas?

Thanks!

[nucacidhunter]

Counting with a hemocytometer.

[austinso]

more context is needed, but you could:

1. use A600 as a measure of particulate carryover within the supernatant
2. pre-bind beads with biotin-fluorescein, then use biotin to displace to get carryover in terms of biotin-binding equivalents using a standard curve (fluorescence reader required)