Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Thermal cycler with variable temperature lid?

    Does this machine exist:, for example, a machine that can change the lid temperature during the course of the reactions? Something tells me that having the lid set to 75C when my reverse transcription reaction is occurring at 45C is not a good idea. In addition, I've been getting lots of adapter dimers and/or technical sequences/artifacts in library prep which I see on the bioanalyzer during my library constructions. Could not having a heat-changing lid be the reason? I am talking about cross all different sample types/library prep kits/depletions.

  • #2
    Hello seqtechno1,

    I'm not aware of such a cycler. But having the lid temperature on 75°C seems to me very low. For all kind of reaction we use 105°C.

    But I'm unsure whether this can lead to your problem, as the higher lid temperature should just prevent vaporescence of your sample.

    fin swimmer

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Latest Developments in Precision Medicine
      by seqadmin



      Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

      Somatic Genomics
      “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
      05-24-2024, 01:16 PM
    • seqadmin
      Recent Advances in Sequencing Analysis Tools
      by seqadmin


      The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
      05-06-2024, 07:48 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 03:16 PM
    0 responses
    13 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 05-29-2024, 01:32 PM
    0 responses
    13 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 05-24-2024, 07:15 AM
    0 responses
    202 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 05-23-2024, 10:28 AM
    0 responses
    223 views
    0 likes
    Last Post seqadmin  
    Working...
    X