Hi. I have my own customized oligos and I would like to anneal them to make adapters. I've seen many protocols (including the Meyer's one) and I am confused whether to use Annealing Buffer with EDTA or without EDTA.
Now I have the oligos in lyophilized form: in which buffer should I resuspend them? H2O or TE or Tris-HCl?
And for Annealing Buffer:
Tris+ NaCl + EDTA? or TrisHCl only?
I will use NEBNext kit to do the ligation. Will EDTA interfere with the ligation process?
Please help. =(
Now I have the oligos in lyophilized form: in which buffer should I resuspend them? H2O or TE or Tris-HCl?
And for Annealing Buffer:
Tris+ NaCl + EDTA? or TrisHCl only?
I will use NEBNext kit to do the ligation. Will EDTA interfere with the ligation process?
Please help. =(
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