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Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Started by samcd, 07-17-2013, 06:33 AM
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3 responses
3,045 views
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by samcd
07-18-2013, 04:18 AM
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Started by elisadouzi, 09-28-2011, 04:09 PM
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3 responses
2,958 views
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by Riva
07-17-2013, 07:32 AM
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Started by pyridine, 07-05-2013, 07:31 AM
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4 responses
3,520 views
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by pyridine
07-17-2013, 03:18 AM
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Started by JNissen, 07-13-2010, 05:38 AM
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6 responses
6,281 views
0 reactions
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by colossus
07-12-2013, 11:10 AM
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Started by weihuameng, 07-08-2013, 06:39 AM
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0 responses
1,512 views
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by weihuameng
07-08-2013, 06:39 AM
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Started by weihuameng, 07-03-2013, 05:07 AM
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1 response
1,083 views
0 reactions
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Last Post
by dpryan
07-08-2013, 01:35 AM
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Started by purplesock42, 01-29-2010, 01:11 PM
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2 responses
3,722 views
0 reactions
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Last Post
by NextGenSeq
07-05-2013, 08:57 AM
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Started by shurjo, 07-14-2011, 01:38 PM
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2 responses
3,836 views
0 reactions
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Last Post
by luc
07-04-2013, 03:30 PM
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Started by ts1127, 07-03-2013, 05:57 AM
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1 response
1,701 views
0 reactions
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Last Post
by jinpingzhang
07-03-2013, 06:25 AM
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Started by microgirl123, 07-02-2013, 09:25 AM
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1 response
1,598 views
0 reactions
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Last Post
by kwaraska
07-02-2013, 11:45 AM
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by SEQadmin2
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...-
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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07-01-2026, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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Started by SEQadmin2, Yesterday, 11:05 AM
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Yesterday, 11:05 AM
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Started by SEQadmin2, 07-02-2026, 11:08 AM
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07-02-2026, 11:08 AM
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Started by SEQadmin2, 06-30-2026, 05:37 AM
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06-30-2026, 05:37 AM
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