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Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Started by dasi, 03-12-2026, 08:38 AM
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by dasi
03-12-2026, 08:38 AM
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Started by alannathomas, 11-28-2025, 03:16 AM
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247 views
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by alannathomas
11-28-2025, 03:16 AM
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Started by CitizenSnips, 09-08-2025, 06:13 AM
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164 views
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by CitizenSnips
09-08-2025, 06:13 AM
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Started by AngelaT, 09-05-2025, 12:44 PM
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381 views
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by AngelaT
09-05-2025, 12:44 PM
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Started by BioDynami, 01-05-2023, 02:13 PM
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1 response
1,277 views
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by zaredmarch
07-07-2025, 04:54 AM
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Started by AngelaT, 12-18-2024, 07:35 AM
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1 response
209 views
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by jiggydancer
06-09-2025, 05:11 AM
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Started by bmseverance, 04-16-2025, 07:11 AM
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210 views
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by bmseverance
04-16-2025, 07:11 AM
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Started by RemitoAmigo, 09-04-2014, 11:27 AM
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8 responses
4,645 views
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Estimating the Relative Abundance of a Species in a Mixed Microbial Community Using 16S PCR
by Ljrdain2508
Started by Ljrdain2508, 02-25-2025, 01:06 PM
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84 views
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by Ljrdain2508
02-25-2025, 01:06 PM
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Started by irate.pirate, 02-20-2025, 04:32 PM
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162 views
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by irate.pirate
02-20-2025, 04:32 PM
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by SEQadmin2
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...-
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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07-01-2026, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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Started by SEQadmin2, 07-02-2026, 11:08 AM
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07-02-2026, 11:08 AM
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