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Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Started by bcf, 01-06-2011, 11:52 AM
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1 response
2,698 views
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by bcf
02-12-2011, 05:43 PM
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Started by CG&R, 12-16-2010, 07:01 AM
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3 responses
4,343 views
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by CG&R
02-10-2011, 02:00 AM
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Started by sarjo, 02-07-2011, 07:28 AM
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7 responses
4,391 views
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by sarjo
02-09-2011, 01:10 PM
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Started by sinapius, 02-09-2011, 12:39 PM
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0 responses
3,221 views
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by sinapius
02-09-2011, 12:39 PM
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Started by pmiguel, 02-09-2011, 08:47 AM
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0 responses
1,787 views
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by pmiguel
02-09-2011, 08:47 AM
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Started by Sebastian123, 02-04-2011, 07:41 AM
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3 responses
2,278 views
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by Sebastian123
02-09-2011, 04:10 AM
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Started by VanessaS, 01-25-2011, 03:31 PM
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1 response
3,154 views
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by Jessica_L
02-04-2011, 09:06 AM
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Started by Dapip33, 02-01-2011, 07:49 PM
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6 responses
5,923 views
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by Dapip33
02-04-2011, 08:11 AM
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Started by Ulukai, 02-03-2011, 12:34 PM
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0 responses
2,408 views
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by Ulukai
02-03-2011, 12:34 PM
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Started by yuhangzhang, 11-19-2010, 08:23 AM
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0 responses
3,337 views
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Last Post
by davidmason
01-31-2011, 12:10 PM
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by SEQadmin2
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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07-01-2026, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
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