Does anyone know any software that will close gaps between contigs using Solexa mate-pair data (insertion ~2500kb)?
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Are you saying u can generate reads with an insert size of 2500kb? -
Woops! 2500b
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MComment
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We do...
At the Sanger Insitute, we developed some software which is not published yet for filling the gaps using solexa reads. Is working really well, and it let you close around 60% of the gaps (in average). We haven't test it using that length of insert size but the projections tell us that it would close more gaps with larger insert sizes.
Also we have things for error correction that uses solexa reads too.
Contact me if you want more details.Comment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
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