Hi everyone!
I am quite new to this field, and as such have a lot of questions but I don't know how to frame it...so please excuse me as I display my ignorance
I have the clc genomic workbench software and I plan to use it to analyze some data from a fly (insect)species. This species isn't studied a lot yet, thus there is no reference genome to compare my results with. However I want to compare it with insect species that already have a reference genome eg D.Melanogaster.
My question is, what steps should I take (using clc's features) from beginning to end so as to reach the step of looking for the variants? I know clc has features like trim sequences, mapping, de novo assembly, variant detection, annotating variants etc....
Any ideas/suggestions will be much appreciated
I am quite new to this field, and as such have a lot of questions but I don't know how to frame it...so please excuse me as I display my ignorance
I have the clc genomic workbench software and I plan to use it to analyze some data from a fly (insect)species. This species isn't studied a lot yet, thus there is no reference genome to compare my results with. However I want to compare it with insect species that already have a reference genome eg D.Melanogaster.
My question is, what steps should I take (using clc's features) from beginning to end so as to reach the step of looking for the variants? I know clc has features like trim sequences, mapping, de novo assembly, variant detection, annotating variants etc....
Any ideas/suggestions will be much appreciated