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  • prettyPeas
    Junior Member
    • Jun 2011
    • 1

    #1

    Error in cdna for newMapping

    I followed the following directions for aligning 454 reads to an assembled rna-seq dataset.
    newMapping projdir
    addRun [options: -p, -lib, -mcf] [projdir] filedesc
    removeRun [projdir] filedesc
    setRef [options: -gref, -cref, -random] [projdir] refdesc
    runProject [options] [projdir]

    I got the following error when I tried to use the -cref option with setRef
    my command:
    setRef -cref DH1 /fullpathname/grossSilber.fa
    error:
    Error: File is not a fasta file of sequence: -cref

    Is there a particular format for a cref fasta?

    It worked when I setRef with no options, but of course runProject won't work with -cdna

    Any helpful hints?
  • flxlex
    Moderator
    • Nov 2008
    • 412

    #2
    Originally posted by prettyPeas View Post
    I got the following error when I tried to use the -cref option with setRef
    my command:
    setRef -cref DH1 /fullpathname/grossSilber.fa
    error:
    Error: File is not a fasta file of sequence: -cref
    I am pretty sure the problem is the 'DH1' between the -cref and the path. -cref expects only a file, or the path to a file.

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