1/4th of microtitre plate enough to characterize transcriptome of non-model legume?
Dear Members,
In your experience, do you think 1/4th of a microtitre plate is enough to characterize the genome of a non-model legume species?
The 454 sequencing performed in the above fashion only resulted in a few hundred thousand reads of a not-so great coverage.
I was under the impression that,to fully characterize a transcriptome, one must sequence several samples of many genotypes in illumina and 454, combine the data and assemble, annotate etc, to achieve a good quality transcriptome that may represent the species.
What do you think?
In your experience, do you think 1/4th of a microtitre plate is enough to characterize the genome of a non-model legume species?
The 454 sequencing performed in the above fashion only resulted in a few hundred thousand reads of a not-so great coverage.
I was under the impression that,to fully characterize a transcriptome, one must sequence several samples of many genotypes in illumina and 454, combine the data and assemble, annotate etc, to achieve a good quality transcriptome that may represent the species.
What do you think?