Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • engencore
    Member
    • Mar 2009
    • 10

    #1

    OTF Ti signal processing

    Last week, we ran our first Ti plate with the signal processing during the run (vs. doing only image collection then signal afterwards). The images were all collected properly and looked likel signal processing was fine until ~step 130 where it caused the SW to crash and kick out to the adminrig login screen with the red flashing light. I logged back in and took the images and did runAnalysisPipe and everything was fine. Any similar experience for others?
  • maubp
    Peter (Biopython etc)
    • Jul 2009
    • 1544

    #2
    What version of the Newbler pipeline do you have?

    Comment

    • engencore
      Member
      • Mar 2009
      • 10

      #3
      we're running 2.0.01.12. We've recently noticed too that the signal processing is not completing (i.e. no Wells tab/information) so we have to do the signal processing on the datarig (same SW but no problems).

      Comment

      • maubp
        Peter (Biopython etc)
        • Jul 2009
        • 1544

        #4
        I was hoping someone else would reply with a more authoritative answer. However, I have a vague recollection from a local next gen meeting of someone mentioning this was a known issue with a recent Newbler update (hence my question about which version you were using).

        I'd suggest you check with your Roche contact to see if this is the case.

        Comment

        Latest Articles

        Collapse

        • SEQadmin2
          Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
          by SEQadmin2



          CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

          Despite this, “CRISPR helped turn genome editing from a specialized technique into
          ...
          07-31-2026, 11:01 AM
        • SEQadmin2
          Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
          by SEQadmin2


          Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

          The systematic characterization of the human proteome has
          ...
          07-20-2026, 11:48 AM
        • SEQadmin2
          Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
          by SEQadmin2



          Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
          ...
          07-09-2026, 11:10 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, Yesterday, 07:41 AM
        0 responses
        11 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 08-03-2026, 10:13 AM
        0 responses
        25 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 07-31-2026, 02:55 AM
        0 responses
        38 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 07-24-2026, 12:17 PM
        0 responses
        25 views
        0 reactions
        Last Post SEQadmin2  
        Working...