Last week, we ran our first Ti plate with the signal processing during the run (vs. doing only image collection then signal afterwards). The images were all collected properly and looked likel signal processing was fine until ~step 130 where it caused the SW to crash and kick out to the adminrig login screen with the red flashing light. I logged back in and took the images and did runAnalysisPipe and everything was fine. Any similar experience for others?
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I was hoping someone else would reply with a more authoritative answer. However, I have a vague recollection from a local next gen meeting of someone mentioning this was a known issue with a recent Newbler update (hence my question about which version you were using).
I'd suggest you check with your Roche contact to see if this is the case.
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
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