Three protocol PDFs attached that I stumbled upon on the web, I thought some of you may be interested.
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
This is a sticky topic.
X
X
-
cDNA from Evrogen with Ti
Has anybody had any experience with Ti sequencing Evrogen Trimmer Direct cDNA? For a 2 region Ti, we got 67 Mb and only 20% pass. The support people feel it is a problem in the emulsion PCR. Any help would be much appreciated. We've sequenced ~30-40 similar samples with FLX and no problems (over 100 Mb, ~440,000 reads per run).
Comment
-
Hello, I´m a new member.
Originally posted by ECO View PostThree protocol PDFs attached that I stumbled upon on the web, I thought some of you may be interested.
Thank you for the three protocol pdf!Last edited by futuro_cortina; 03-09-2009, 11:21 AM.
Comment
-
PTS Wisdom?
Hi
We are currently working on de novo RNA-seq and re-sequencing in a pre-genome species (onion) and are interested in PTS or a similar generic barcoding approach to enable us to do population genetic studies.
If anyone can give me any opinion or advice concerning this approach I'd be really grateful. To date we are just working with barcoded primers
Regards
John
Comment
-
Hi
We are trying to work out cDNA sequencing using 454, but Titanium did not work, and Standard was severely affected by polyA tail despite the nice profile of the sstDNA library.
We used Dynabeads for mRNA purification, and Stratagen Just cDNA kit for ds cDNA synthesis (1st strand primed by none-anchored oligo dT from the kit). Our samples tend to have long polyA tail, and we found 454 sequencing cannot tolerate this.
We are trying anchored dT primer now, but would appreciate any suggestion, such as choice of kit, 1st strand priming method (dT vs random primer?), or any tips in improving library constrcution for greater chance of success in sequencing?
Thank you.
Comment
-
-
re Poly A etc
We have had very nice results from Ti sequencing of normalised cDNA prepared using Evrogen Mint + Trimmer kits, using a modified 3' Primer XXXXXXXXXXX(T)4G(T)9C(T)10VN as described by Beldade et al.
The information posted at http://www.cees.uio.no/research/faci...equencing.html was very useful.
Comment
Latest Articles
Collapse
-
by seqadmin
While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...-
Channel: Articles
Today, 07:15 AM -
-
by seqadmin
Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.
Somatic Genomics
“We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...-
Channel: Articles
05-24-2024, 01:16 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, Today, 08:18 AM
|
0 responses
8 views
0 likes
|
Last Post
by seqadmin
Today, 08:18 AM
|
||
Started by seqadmin, Today, 08:04 AM
|
0 responses
10 views
0 likes
|
Last Post
by seqadmin
Today, 08:04 AM
|
||
Started by seqadmin, 06-03-2024, 06:55 AM
|
0 responses
13 views
0 likes
|
Last Post
by seqadmin
06-03-2024, 06:55 AM
|
||
Started by seqadmin, 05-30-2024, 03:16 PM
|
0 responses
27 views
0 likes
|
Last Post
by seqadmin
05-30-2024, 03:16 PM
|
Comment