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  • Illumina Primers

    Hi everyone,

    I am new to the SEQanswers community and have been searching through
    the threads to find primer and adapter concentrations for use with the Illumina GA II system.

    I am planning to do MeDIP-Seq for Paired-end multiplexed (index/tagged) samples.

    In some of the other threads it is implied that the sequencing primers are 100um. However does this concentration apply to the adapters or the primers for LM-PCR ??

    Also another thing has me slightly confused.
    Some groups achieve multiplexing by designing tagged adapters, whereas others add the tag during LM-PCR. Is this because it's not too important when the tag goes in or are there specific advantages to either strategy.

    Considered thoughts would be greatly appreciated.

    Cheers

    Will

  • #2
    Never mind !

    I have been shown the error of my ways

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