Hi all,
Could anybody give me an idea or though on the following?
I made a run with the MiSeq using the TrueSeq LT kit and 151 cycles. It was an amplicon library with 3 different DNA, everyone with his own index. The run has very low Q30, around 6%, but has Clusters PF (passing filter) of 85%. The number of clusters is around 1100 K/mm2. Moreover, no reads were identified.
Could have been the PhiX? it was set at 15% but not freshly made, only two weeks old (protocol recommendation is 3 weeks max).
Anyway, any help will be greatly appreciated. Bye.
Could anybody give me an idea or though on the following?
I made a run with the MiSeq using the TrueSeq LT kit and 151 cycles. It was an amplicon library with 3 different DNA, everyone with his own index. The run has very low Q30, around 6%, but has Clusters PF (passing filter) of 85%. The number of clusters is around 1100 K/mm2. Moreover, no reads were identified.
Could have been the PhiX? it was set at 15% but not freshly made, only two weeks old (protocol recommendation is 3 weeks max).
Anyway, any help will be greatly appreciated. Bye.
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