Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • demultiplexing with dual indexing

    Hi all, I am trying ascertain that my libraries can be demultiplexed. What we have are 6 samples prepared with the Nextera XT kit (whole genome shotgun). They each have unique i7 indexes, but all have the same i5 index. Even if read1 and read2 do not overlap, the samples should still demultiplex fine, right? Probably a silly question, but I just want to be certain....
    JB

  • #2
    Yes, demultiplexing is unrelated to whether reads overlap.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Understanding Genetic Influence on Infectious Disease
      by seqadmin




      During the COVID-19 pandemic, scientists observed that while some individuals experienced severe illness when infected with SARS-CoV-2, others were barely affected. These disparities left researchers and clinicians wondering what causes the wide variations in response to viral infections and what role genetics plays.

      Jean-Laurent Casanova, M.D., Ph.D., Professor at Rockefeller University, is a leading expert in this crossover between genetics and infectious...
      09-09-2024, 10:59 AM
    • seqadmin
      Addressing Off-Target Effects in CRISPR Technologies
      by seqadmin






      The first FDA-approved CRISPR-based therapy marked the transition of therapeutic gene editing from a dream to reality1. CRISPR technologies have streamlined gene editing, and CRISPR screens have become an important approach for identifying genes involved in disease processes2. This technique introduces targeted mutations across numerous genes, enabling large-scale identification of gene functions, interactions, and pathways3. Identifying the full range...
      08-27-2024, 04:44 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Today, 06:25 AM
    0 responses
    13 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, Yesterday, 01:02 PM
    0 responses
    12 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 09-18-2024, 06:39 AM
    0 responses
    14 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 09-11-2024, 02:44 PM
    0 responses
    14 views
    0 likes
    Last Post seqadmin  
    Working...
    X