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  • jwfoley
    Senior Member
    • Jun 2009
    • 183

    #1

    phiX on Bioanalyzer

    In case anyone else was curious. This is Illumina's ΦX174 reference library ("PhiX Sequencing Control V3", p/n FC-110-3001, allegedly 10 nM), undiluted. As you can see, it's fairly high-MW (460 bp), so smaller libraries might cluster preferentially, and it's actually 8 nM.
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  • jlei_face
    Member
    • Oct 2014
    • 10

    #2
    Thanks for sharing this data.
    I'd just like to add that concentrations of PhiX vary from lot to lot so 8nM wouldn't necessarily be applicable to every tube.

    Comment

    • ritujasaxena
      Junior Member
      • Apr 2016
      • 1

      #3
      Thanks for the info..

      Yes the concentration varies a lot.

      I have observed it to be between 8-14 nM in different cases.

      Comment

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