Hi all,
I am just wondering why the # of DNA copies in each cluster is capped between 1000-2000.
Is this just due to the size of the well? I'm guessing that all fixed oligos in a well are extended during bridge amplification.
Or does the sequencing process fail w/ too many copies?
Thanks.
Jim
I am just wondering why the # of DNA copies in each cluster is capped between 1000-2000.
Is this just due to the size of the well? I'm guessing that all fixed oligos in a well are extended during bridge amplification.
Or does the sequencing process fail w/ too many copies?
Thanks.
Jim
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