We recently performed a sequencing run on the MiSeq sequencer using a V3 (600 cycle kit).
With regards to the per tile quality plot (obtained when using the FastQC analysis software) for one of our samples, it seems as though there is a large area that is flagged in red for R2, indicating that those tiles had worse qualities.
I was just wondering on possible reasons why this would happen and how it could be prevented in future runs?
Thanks!
With regards to the per tile quality plot (obtained when using the FastQC analysis software) for one of our samples, it seems as though there is a large area that is flagged in red for R2, indicating that those tiles had worse qualities.
I was just wondering on possible reasons why this would happen and how it could be prevented in future runs?
Thanks!
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