Hi everyone,
I am following Illumina's 16S protocol for sequencing and I use Nextera XT Index v2 adapters kit. I do my runs on MiSeq using 500 cycle v2 kit. The maximum number of samples I have sequenced in a same run is 64.
Currently, I am doing budget planning for a new project that would include almost ~240 samples. I prefer to sequence them all in the same run. The Nextera XT v2 index kit comes in 4 sets (A,B,C and D). Is it possible just to buy set A and D and use them together for the maximum theoretical limit of 384 samples or do I have to buy all 4 sets for that purpose?
To elaborate a bit, on a 96 well plate outlay there are 8 rows (S (i5) adapters) and 12 columns (N (i7) adapters). Illumina sells the Nextera XT v2 adapters in a combinatorial permutation: Set A (S1,N1), Set B (S1,N2), Set C (S2,N1) and set D (S2,N2), where Sx is 8 adapters and Nx is 12 adapters as found on this pdf: https://support.illumina.com/content...0002694-01.pdf
The information about the sets is here https://www.illumina.com/products/by...ra-xt-dna.html
Your help is appreciated.
I am following Illumina's 16S protocol for sequencing and I use Nextera XT Index v2 adapters kit. I do my runs on MiSeq using 500 cycle v2 kit. The maximum number of samples I have sequenced in a same run is 64.
Currently, I am doing budget planning for a new project that would include almost ~240 samples. I prefer to sequence them all in the same run. The Nextera XT v2 index kit comes in 4 sets (A,B,C and D). Is it possible just to buy set A and D and use them together for the maximum theoretical limit of 384 samples or do I have to buy all 4 sets for that purpose?
To elaborate a bit, on a 96 well plate outlay there are 8 rows (S (i5) adapters) and 12 columns (N (i7) adapters). Illumina sells the Nextera XT v2 adapters in a combinatorial permutation: Set A (S1,N1), Set B (S1,N2), Set C (S2,N1) and set D (S2,N2), where Sx is 8 adapters and Nx is 12 adapters as found on this pdf: https://support.illumina.com/content...0002694-01.pdf
The information about the sets is here https://www.illumina.com/products/by...ra-xt-dna.html
Your help is appreciated.
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