Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Adapters sequence

    Hi all!
    Can't find the adaptors sequence for Amplicon Sequencing)) Can smb help me?

    thanks!)))

  • #2
    maybe you should start specifying which technology you're using on which instrument...

    Comment


    • #3
      Ion Torrent, Amplicon sequencing))

      Comment


      • #4
        Assuming it's Ion Torrent as it's in the Ion Torrent thread...
        Attached Files

        Comment


        • #5
          In this manual are only the primer sequence, ... but adaptor sequence absence? is they full complementary and same size?
          Last edited by Chartiza; 02-01-2012, 03:53 AM.

          Comment


          • #6
            The PCR creates the adapter sequences using the P1 and A tags on the site specific primers.

            Comment


            • #7
              having the sequence of the (Y) adaptors would be handy!

              Comment


              • #8
                What's interesting is that these sequences seem to be a mash up of the 454 and Solid sequences. There is even some part complimentary sequence between the forward and reverse primer sequences. Surely this is would promote primer-dimer?

                Integrated electronic detection of protons released upon base incorporation. (Life Technologies)

                Comment

                Latest Articles

                Collapse

                • seqadmin
                  Strategies for Sequencing Challenging Samples
                  by seqadmin


                  Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
                  03-22-2024, 06:39 AM
                • seqadmin
                  Techniques and Challenges in Conservation Genomics
                  by seqadmin



                  The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

                  Avian Conservation
                  Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
                  03-08-2024, 10:41 AM

                ad_right_rmr

                Collapse

                News

                Collapse

                Topics Statistics Last Post
                Started by seqadmin, Yesterday, 06:37 PM
                0 responses
                10 views
                0 likes
                Last Post seqadmin  
                Started by seqadmin, Yesterday, 06:07 PM
                0 responses
                9 views
                0 likes
                Last Post seqadmin  
                Started by seqadmin, 03-22-2024, 10:03 AM
                0 responses
                51 views
                0 likes
                Last Post seqadmin  
                Started by seqadmin, 03-21-2024, 07:32 AM
                0 responses
                67 views
                0 likes
                Last Post seqadmin  
                Working...
                X