I think i used to use 4.2.2. But i don't have the archive to install it (i didn't install it on our cluster).
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Hi,
I just want to reiterate how crazy double encoding is! Thought we were having problems with our aligner as the 'reads' weren't mapping to the reference. Why on earth did ABI pick those 4 letters? Why even double encode in the first place?!
Thanks Rick!
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SeqAA - agree.
People, if you want to analyse Solid data properly use colour space. If you're forced into the dark arts of base space conversion i.e. for de novo assembly I would strongly recommend reading the supplements of this paper:
Iverson et al. 2012, Science : Untangling genomes from metagenomes ....
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...-
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
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