Hi!
Does anybody have experience with doing manual enrichment of the templated beads (for SOLiD 5500) in this post-EZ period?
As we aim to get the wíldfire there isnt much point in spending a lot of money on the EZ bead system, and according to ABI (when we bought the system) manual bead prep is still doable (all though they did advise that its to much work).
So we now have beads from a perfect ePCR run, and want to enrich them.
Here comes the question(s):
Does anyone know how to apply the EZ bead enrichment kit consumables for the manual enrichment protocol?
Is anybody aware of how and where to buy the manual enrichment reagents?
Has anybody tried applying the original Polonator templated-bead enrichment protocol on SOLiD beads?
Desperately hope somebody can help on this!!!!!
Best greetings,
Does anybody have experience with doing manual enrichment of the templated beads (for SOLiD 5500) in this post-EZ period?
As we aim to get the wíldfire there isnt much point in spending a lot of money on the EZ bead system, and according to ABI (when we bought the system) manual bead prep is still doable (all though they did advise that its to much work).
So we now have beads from a perfect ePCR run, and want to enrich them.
Here comes the question(s):
Does anyone know how to apply the EZ bead enrichment kit consumables for the manual enrichment protocol?
Is anybody aware of how and where to buy the manual enrichment reagents?
Has anybody tried applying the original Polonator templated-bead enrichment protocol on SOLiD beads?
Desperately hope somebody can help on this!!!!!
Best greetings,
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