Dear all,
After having used the Targetseq Exome Capture kit for years, LifeTech provided us with a new version of the kit called Ion TargetSeq™ Exome Enrichment kit for the exome capture of the libraries. In my first Solid 5500XL run using it, i only got circa 20-25% of exomic sequences, instead of the 75% of "read on target" that i usually get with the former kit.
Would anyone have an idea of what could be the cause? Did you already used this kit and what results did you get? Is there any tricky change in the way you do perform the capture with this new version and that would not be mentionned in the procotol?
My samples were FFPE; i'm wondering if the formalin and/or paraffin could interfer with a step of the library or the capture and could dramatically reduce its yeld...
Thank you,
durandg.
After having used the Targetseq Exome Capture kit for years, LifeTech provided us with a new version of the kit called Ion TargetSeq™ Exome Enrichment kit for the exome capture of the libraries. In my first Solid 5500XL run using it, i only got circa 20-25% of exomic sequences, instead of the 75% of "read on target" that i usually get with the former kit.
Would anyone have an idea of what could be the cause? Did you already used this kit and what results did you get? Is there any tricky change in the way you do perform the capture with this new version and that would not be mentionned in the procotol?
My samples were FFPE; i'm wondering if the formalin and/or paraffin could interfer with a step of the library or the capture and could dramatically reduce its yeld...
Thank you,
durandg.
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