Hi all,
Since I am new here, I am not familiar with the data analysis from SOLiD platform.
Yesterday, I was told to cut the reads(50bp) into 30bp or shorter before performing mapping, otherwise I won't get anything since almost half of the read is from adaptor.
So, could you give me some suggestions about which software can do this work and what the adaptor should I prepare before?
Thanks a lot.
BTW, I am focus on miRNA sequencing. And yesterday I used PerM to mapping the reads with the genome reference, but I only got 0.03% mapping result.
Since I am new here, I am not familiar with the data analysis from SOLiD platform.
Yesterday, I was told to cut the reads(50bp) into 30bp or shorter before performing mapping, otherwise I won't get anything since almost half of the read is from adaptor.
So, could you give me some suggestions about which software can do this work and what the adaptor should I prepare before?
Thanks a lot.
BTW, I am focus on miRNA sequencing. And yesterday I used PerM to mapping the reads with the genome reference, but I only got 0.03% mapping result.
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