The purpose is to annotate de-novo bacteria genome assembly. I see there are many tools, like GeneMark, RAST, etc. Does any one have any recommendation, for de-novo annotation, and comparative annotation, respectively? Thanks a lot.
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For individual bacterial genomes NCBI Prokaryotic Genome Annotation Pipeline: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/ -
Thanks! But it seems that only when I submit genomes to GenBank I can request annotation by this pipeline. Could I use this pipeline to analyze my data independent of submission?Originally posted by GenoMax View PostComment
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I've used Artemis for annotation once... works very well and has some handy features that facilitates all the process.
Take a look at http://www.sanger.ac.uk/resources/software/artemis/Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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