Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Tophat 2.0.6, empty insertions and deletion bed files

    I've been using Tophat version 2.0.6 for a while and it seemed to work fine. However, today I wanted to use the insertions.bed and deletions.bed files but these files seemed to be empty after Tophat has finished. In previous versions of Tophat it worked fine.

    Does anyone experience the same problem or does anybody know how to fix this problem?

  • #2
    I encountered the same thing in the new version. It was working fine in the previous version. Did you find a fix for that?

    Comment


    • #3
      No I haven't found a fix for that yet. I'm going to run a sample with the option --keep-tmp. There should be some temporary files being created regarding the indels.
      In tophat 2.0.3. it was working fine, but since then these files are empty. I've tried the newest version 2.0.7, but this version doesn't output the indel files either.

      Comment


      • #4
        Even though by keeping the option --keep-tmp, yet I am encountering the unavailability of bed files...

        Comment


        • #5
          Yes, same here, haven't really looked into it anymore. It looks like the segment_juncs step does report empty files:

          Reported 8021 total potential splices
          Reporting 0 potential deletions...
          Reporting 0 potential insertions...
          Reporting potential fusions...

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Essential Discoveries and Tools in Epitranscriptomics
            by seqadmin


            The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
            Today, 07:01 AM
          • seqadmin
            Current Approaches to Protein Sequencing
            by seqadmin


            Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
            04-04-2024, 04:25 PM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, 04-11-2024, 12:08 PM
          0 responses
          37 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-10-2024, 10:19 PM
          0 responses
          41 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-10-2024, 09:21 AM
          0 responses
          35 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-04-2024, 09:00 AM
          0 responses
          54 views
          0 likes
          Last Post seqadmin  
          Working...
          X