Hey all, just a quick question regarding Illumina's TruSeq DNA sample prep protocol. After adapter ligation, there's a cleanup step with Ampure XP. If you're doing the gel-cut size-selection step though, that step comes directly afterwards. The manual seems to suggest that you need to do the Ampure cleanup even if you're planning to do the gel-cut afterwards, but I feel like electrophoresis should remove any contaminants well enough on its own.
Has anyone tried skipping straight to electrophoresis without the Ampure cleanup? Is there any reason that shouldn't work?
Has anyone tried skipping straight to electrophoresis without the Ampure cleanup? Is there any reason that shouldn't work?
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