Illumina Tru Seq kit v2

[qob]

I was moving the Illumina Tru Seq kit v2 (http://www.biotech.wisc.edu/Librarie...15026495_C.pdf) components to another lab and somehow the RNA binding beads got put in the freezer and not the fridge. I do not imagine anyone else has done anything so stupid, but I just wanted to know what everyone thinks I should do as Illumina do not sell the beads separately and buying an entire new kit is out of the question.

So should I:
use the beads that have been frozen or
buy an alternative bead type, e.g. Invitrogen dynabeads and then elute using Illumina's buffer?

We have plenty of our RNA samples so this is not a limiting factor.

Thanks.

[pmiguel]

I was moving the Illumina Tru Seq kit v2 (http://www.biotech.wisc.edu/Librarie...15026495_C.pdf) components to another lab and somehow the RNA binding beads got put in the freezer and not the fridge. I do not imagine anyone else has done anything so stupid, but I just wanted to know what everyone thinks I should do as Illumina do not sell the beads separately and buying an entire new kit is out of the question.

So should I:
use the beads that have been frozen or
buy an alternative bead type, e.g. Invitrogen dynabeads and then elute using Illumina's buffer?

We have plenty of our RNA samples so this is not a limiting factor.

Thanks.

I strongly recommend you call Illumina and beg them to replace that component. They are usually pretty good about this.

--
Phillip