Hello, I am new to using Next Generation Sequencing.
I am hoping to do long range PCR (20-30 kb), followed by library prep with a Nextera XT kit, followed by sequencing with a MiSeq.
My PI has asked me to find a PCR enzyme that is appropriate for long PCR and will yield products that are appropriate for the next steps of my project. I'm not sure what criteria to look for in a good enzyme for this situation. Is there anything I should be on the look out for? In particular, he told me to look at the three enzymes offered by Life Technologies on this page http://www.lifetechnologies.com/us/e...gment-pcr.html and determine which one was the best for this purpose. I have already called the company, and they were not terribly helpful.
If anyone has suggestions, I would be very happy to hear them. If you could put them in as simple/basic language as possible, I would appreciate that, because I am new to this field.
Thanks so much!
I am hoping to do long range PCR (20-30 kb), followed by library prep with a Nextera XT kit, followed by sequencing with a MiSeq.
My PI has asked me to find a PCR enzyme that is appropriate for long PCR and will yield products that are appropriate for the next steps of my project. I'm not sure what criteria to look for in a good enzyme for this situation. Is there anything I should be on the look out for? In particular, he told me to look at the three enzymes offered by Life Technologies on this page http://www.lifetechnologies.com/us/e...gment-pcr.html and determine which one was the best for this purpose. I have already called the company, and they were not terribly helpful.
If anyone has suggestions, I would be very happy to hear them. If you could put them in as simple/basic language as possible, I would appreciate that, because I am new to this field.
Thanks so much!
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