Hi all,
We are outsourcing RNA-seq along with RNA isolation from a gram positive bacterium. The service provider has sent the QC report (Tapestation) for the isolated RNA.
The first sample is said to have failed and has the following profile.
For other sample, the RNA isolation is being claimed to have succeeded with the following profiles.
Do you have any suggestions regarding the following points?
(1) Why there is weird peak between 25 and 200 and also a corresponding darkest band in all the samples?
(2) Shall the service provider be asked to proceed with the library preperation for the bottom 3 samples?
Many thanks
We are outsourcing RNA-seq along with RNA isolation from a gram positive bacterium. The service provider has sent the QC report (Tapestation) for the isolated RNA.
The first sample is said to have failed and has the following profile.
For other sample, the RNA isolation is being claimed to have succeeded with the following profiles.
Do you have any suggestions regarding the following points?
(1) Why there is weird peak between 25 and 200 and also a corresponding darkest band in all the samples?
(2) Shall the service provider be asked to proceed with the library preperation for the bottom 3 samples?
Many thanks
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