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I want to do chip-seq for a transcription factor in bacteria for Illumina. I' need help for sample preparation for bacteria since all I find is eukaryotes. Brand new with the technique, sorry!
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I haven't done ChIP in bacteria but once you have the ChIP material I would think making the libraries would be the same. I'm waiting to get back sequencing data from my first ChIP seq libraries. If all looks good then I will be able to give some advice. You could contact Illumina to see if they have any protocols for ChIP in prokayotes. -
A very interesting study shows some of the pitfalls of ChIP applications and offers some advances to published protocols.
The paper is dedicated to ChIP on Chip but most of it also applies to Chip-Seq, too.
If you're new to the tech, I would recommend reading this paper.
Here's the ref: Waldminghaus & Skarstad 2010, BMC Genomics, 11:414Comment
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM
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