Hi All,
We purchased an illumina nextera xt DNA library prep kit and accompanying 96 index kit with the goal of doing single cell RNA-seq on mouse cells following single cell sorting on a fluidigm C1.
For a number of reasons, we put the single cell RNA-Seq project on hold, but still want to use up the nextera xt kit and indexes in our lab.
Has anyone ever used the xt kit to perform any type of bulk RNA-Seq (i.e. not single cell) using eukaryotic cells before? If not does anyone have any tips or suggestions on how to try this?
In the past, we have used the standard nextera kit (not the xt) for bulk RNA-seq of mouse cells, but that is not currently an option.
Thanks
We purchased an illumina nextera xt DNA library prep kit and accompanying 96 index kit with the goal of doing single cell RNA-seq on mouse cells following single cell sorting on a fluidigm C1.
For a number of reasons, we put the single cell RNA-Seq project on hold, but still want to use up the nextera xt kit and indexes in our lab.
Has anyone ever used the xt kit to perform any type of bulk RNA-Seq (i.e. not single cell) using eukaryotic cells before? If not does anyone have any tips or suggestions on how to try this?
In the past, we have used the standard nextera kit (not the xt) for bulk RNA-seq of mouse cells, but that is not currently an option.
Thanks
Comment