Hi all,
Has anybody any advice on the "best" concentration (pM) to load a ddRAD or GBS libraries on a HiSeq 3000/4000 ? ddRAD have 300-600 bp, while GBS more around 250-350 bp lib sizes. I know the longer fragments of ddRAD might create a problem may be with the new chemistry/patterned flowcells.
Also 10% Phix would be ok ?
Cheers,
Greg
Has anybody any advice on the "best" concentration (pM) to load a ddRAD or GBS libraries on a HiSeq 3000/4000 ? ddRAD have 300-600 bp, while GBS more around 250-350 bp lib sizes. I know the longer fragments of ddRAD might create a problem may be with the new chemistry/patterned flowcells.
Also 10% Phix would be ok ?
Cheers,
Greg
Comment