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Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Started by seq_me, 06-30-2011, 09:59 AM
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by seq_me
06-30-2011, 09:59 AM
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Started by DrDTonge, 06-10-2011, 04:36 AM
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6 responses
2,929 views
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by DrDTonge
06-29-2011, 11:10 PM
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Started by GW_OK, 11-29-2010, 01:17 PM
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23 responses
16,127 views
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by ashchin
06-28-2011, 12:06 PM
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Started by joewales, 05-04-2011, 05:41 AM
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5 responses
3,640 views
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by joewales
06-28-2011, 06:43 AM
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Started by Nicky333, 06-24-2011, 05:43 AM
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2,381 views
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by Nicky333
06-24-2011, 05:43 AM
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Started by nikiwilson, 06-17-2011, 01:58 AM
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2 responses
12,202 views
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by NextGenSeq
06-21-2011, 01:36 PM
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Started by Metz, 06-21-2011, 04:05 AM
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2,210 views
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by Metz
06-21-2011, 04:05 AM
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Started by James, 02-11-2011, 04:52 AM
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3 responses
4,375 views
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by RayLangley
06-20-2011, 10:46 AM
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Started by DrDTonge, 06-20-2011, 02:55 AM
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0 responses
1,697 views
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by DrDTonge
06-20-2011, 02:55 AM
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Started by sehrrot, 06-07-2011, 06:12 PM
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4 responses
5,980 views
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Last Post
by sehrrot
06-15-2011, 03:38 PM
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by SEQadmin2
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...-
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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07-01-2026, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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Started by SEQadmin2, Yesterday, 11:05 AM
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by SEQadmin2
Yesterday, 11:05 AM
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Started by SEQadmin2, 07-02-2026, 11:08 AM
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07-02-2026, 11:08 AM
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Started by SEQadmin2, 06-30-2026, 05:37 AM
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by SEQadmin2
06-30-2026, 05:37 AM
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