Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • (newbie question) What is the diff on shotgun method and the other methods?

    As in the title.

    What is the diff on the shotgun sequencing method and other methods (Sanger, or NGS)?

    Thanks.

  • #2
    A bit of searching would have landed you here: http://en.wikipedia.org/wiki/Shotgun_sequencing

    Comment


    • #3
      Originally posted by arkilis View Post
      As in the title.

      What is the diff on the shotgun sequencing method and other methods (Sanger, or NGS)?

      Thanks.
      I think you are confused about some basic terminology. Shotgun is a sequencing strategy. Sanger or NGS are sequencing methodologies (though "NGS" is a generic term which encompasses several different technologies). The shotgun strategy may be carried out using many different methodologies/technologies, including the two you have listed.

      GenoMax is correct. There are several very good introductory tutorials about sequencing strategies and technologies a mere Google search away. It wouldn't be useful to simply repeat here what is already well described elsewhere.
      Last edited by kmcarr; 09-23-2013, 07:54 PM.

      Comment


      • #4
        Originally posted by GenoMax View Post
        A bit of searching would have landed you here: http://en.wikipedia.org/wiki/Shotgun_sequencing
        Thanks, I went to Shotgun wiki page. So many terminologies get me lost there...

        Comment


        • #5
          Originally posted by kmcarr View Post
          I think you are confused about some basic terminology. Shotgun is a sequencing strategy. Sanger or NGS are sequencing methodologies (though "NGS" is a generic term which encompasses several different technologies). The shotgun strategy my be carried out using many different methodologies/technologies, including the two you have listed.

          GenoMax is correct. There are several very good introductory tutorials about sequencing strategies and technologies a mere Google search away. It wouldn't be useful to simply repeat here what is already well described elsewhere.
          Thanks a lot. The answer is quite clear and straightforward.

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Strategies for Sequencing Challenging Samples
            by seqadmin


            Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
            03-22-2024, 06:39 AM
          • seqadmin
            Techniques and Challenges in Conservation Genomics
            by seqadmin



            The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

            Avian Conservation
            Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
            03-08-2024, 10:41 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, Yesterday, 06:37 PM
          0 responses
          10 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, Yesterday, 06:07 PM
          0 responses
          9 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 03-22-2024, 10:03 AM
          0 responses
          50 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 03-21-2024, 07:32 AM
          0 responses
          67 views
          0 likes
          Last Post seqadmin  
          Working...
          X