Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • arkilis
    Senior Member
    • Jul 2013
    • 119

    #1

    (newbie question) What is the diff on shotgun method and the other methods?

    As in the title.

    What is the diff on the shotgun sequencing method and other methods (Sanger, or NGS)?

    Thanks.
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    A bit of searching would have landed you here: http://en.wikipedia.org/wiki/Shotgun_sequencing

    Comment

    • kmcarr
      Senior Member
      • May 2008
      • 1181

      #3
      Originally posted by arkilis View Post
      As in the title.

      What is the diff on the shotgun sequencing method and other methods (Sanger, or NGS)?

      Thanks.
      I think you are confused about some basic terminology. Shotgun is a sequencing strategy. Sanger or NGS are sequencing methodologies (though "NGS" is a generic term which encompasses several different technologies). The shotgun strategy may be carried out using many different methodologies/technologies, including the two you have listed.

      GenoMax is correct. There are several very good introductory tutorials about sequencing strategies and technologies a mere Google search away. It wouldn't be useful to simply repeat here what is already well described elsewhere.
      Last edited by kmcarr; 09-23-2013, 07:54 PM.

      Comment

      • arkilis
        Senior Member
        • Jul 2013
        • 119

        #4
        Originally posted by GenoMax View Post
        A bit of searching would have landed you here: http://en.wikipedia.org/wiki/Shotgun_sequencing
        Thanks, I went to Shotgun wiki page. So many terminologies get me lost there...

        Comment

        • arkilis
          Senior Member
          • Jul 2013
          • 119

          #5
          Originally posted by kmcarr View Post
          I think you are confused about some basic terminology. Shotgun is a sequencing strategy. Sanger or NGS are sequencing methodologies (though "NGS" is a generic term which encompasses several different technologies). The shotgun strategy my be carried out using many different methodologies/technologies, including the two you have listed.

          GenoMax is correct. There are several very good introductory tutorials about sequencing strategies and technologies a mere Google search away. It wouldn't be useful to simply repeat here what is already well described elsewhere.
          Thanks a lot. The answer is quite clear and straightforward.

          Comment

          Latest Articles

          Collapse

          • SEQadmin2
            16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
            by SEQadmin2




            NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
            Today, 06:46 AM
          • SEQadmin2
            Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
            by SEQadmin2



            Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

            Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
            ...
            10-14-2026, 09:55 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by SEQadmin2, 10-28-2026, 09:57 AM
          0 responses
          22 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-28-2026, 09:51 AM
          0 responses
          14 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-22-2026, 11:13 AM
          0 responses
          68 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-22-2026, 10:48 AM
          0 responses
          48 views
          0 reactions
          Last Post SEQadmin2  
          Working...